It has been subject to the Agency's peer and administrative review and has been approved for publication. Mention of trade names or commercial products does not constitute endorse- ment or recommenodation for use. Freeman, the Hazardous Haste Engineering Research Laboratory Work Assignment Manager, whose assistance and support was utilized throughout the program. The authors also extend thanks to other members of the HWERL staff for their assistance and to the many industrial representatives who provided design, operating, and performance data for the waste treatment technologies.
Search World Journal of Microbiology and Biotechnology v. This paper reports the isolation from soil of Penicillium strain PY-1 with strong antagonistic activity against plant pathogenic fungi.
On the basis of its morphological characteristics and the sequence of the ITS region, strain PY-1 was identified as P. The antifungal substances were extracted with ethyl acetate and further separated by high-performance liquid chromatography HPLC ; at least two active components were discovered.
The ability to control plant disease with strain PY-1 was confirmed with S. The potential of strain PY-1 for identifying new antibiotics to control fungal disease and for biological control of plant disease, for example oilseed rape stem rot, is discussed.
Penicillium oxalicum ; Antagonism; Antibiotic; Antifungal substance; Biological control; Sclerotinia sclerotiorum Production and characterization of a glycolipid biosurfactant from Bacillus megaterium using economically cheaper sources by R.
Criteria selected for screening of biosurfactant production by Bacillus megaterium were hemolytic assay, bacterial cell hydrophobicity and the drop-collapse test. The data on hemolytic activity, bacterial cell adherence with crude oil and the drop-collapse test confirmed the biosurfactant-producing ability of the strain.
Experiment 1: Two Base Extraction Jeremy Wolf. 6/30/ TA: Stefanie Lenz We converted both benzoic acid and 2-naphthol to their conjugate bases, which are soluble in water, in two separate steps, with two separate bases. Some extract was lost because it came out of solution and some was lost when the liquid would drip down the mouth. Bioconversion of lignocellulosic biomass to xylitol: An overview cooking, liquid–solid extraction, and filtration in a single step. The most commonly observed phenolic derivatives in the lignocellulosic acid hydrolysate include 4-hydroxy benzoic acid, ferulic acid, and guaiacol (Mussatto and Roberto, ). Experiment Methods and Materials The apparatus used in the experiment include conical flask, 4 decimal place balance, Pasteur pipette, burette, stirrer, dropper, beaker, stand and clamp, filter funnel, filter paper, spatula, kerosene, water and benzoic acid/5(3).
Laboratory scale biosurfactant production in a fermentor was done with crude oil and cheaper carbon sources like waste motor lubricant oil and peanut oil cake, and the highest biosurfactant production was found with peanut oil cake. Characterization of partially purified biosurfactant inferred that it was a glycolipid with emulsification potential of waste motor lubricant oil, crude oil, peanut oil, diesel, kerosene, naphthalene, anthracene and xylene.
Analyses of molecular polymorphisms in a selected set of Calothrix strains, using primers based on repetitive sequences in the genome, led to the unambiguous differentiation of the strains as well as understanding of their genetic relationships.
Seventeen 10 mer random primers were used singly and twelve dual primer combinations were used to examine the phylogenetic relatedness amongst the strains using RAPD- PCR.
A total of nine hundred distinct polymorphic DNA fragments bandsranging from 0. A combination of twelve sets of primers generated nine hundred three distinct polymorphic DNA fragments bandsranging from 0.
The combined analysis of single and multiplex primer combination showed a maximum correlation coefficient of 0. These two isolates belonged to same geographical location. The study undertaken has revealed extensive evidence for the applicability of RAPD in cyanobacterial taxonomy, and furthermore, clearly demonstrated the superior discriminative power of RAPD towards the differentiation of geographically unrelated Calothrix strains.
The strain growth was analyzed by monitoring the viable cell counts c. The analysis of the viable cell counts showed that the medium that most favored bacterial growth was not the one that favored EPS production.
The control medium GYM was the one that most favored the strain growth, at the maximum specific growth rate of 0. Differences in bacterial growth when cultured at three different concentrations of molasses were not observed.
Production of EPS, in all culture media used, began during the exponential phase and continued during the growth stationary phase.
A different result was observed in pattern medium, which presented EPS made up of higher percentage of capsular EPS Industrial liquid-liquid extraction most often involves processing liquid extraction in bioprocessing is the Baniel process for the recovery two immiscible or partially miscible liquids in the form of a disper- of citric acid from fermentation broth with tertiary amine extractants sion of droplets of one liquid (the dispersed phase) suspended.
Goal: The goal of this lab is to separate benzoic acid from a mixture containing benzoic acid, cellulose (a natural polymer of glucose) and methyl orange (a common acid/base indicator).
You will use a chemically active extraction to convert the water insoluble benzoic acid into its water soluble salt by treating the carboxylic acid with base. Liquid-liquid extraction has found large appli cations in the s eparation indust ry because of the mild process co nditions ap plied during t he process i.e.
lo w temperat ure. Weigh g of benzoic acid and transfer it to a mL beaker containing mL water. Stir for 15mins. Filter the solution using pre-weighed filter paper and set aside filtrate for the following procedure.
Tert-butyl alcohol is an isomer of butanol that contains a tertiary butyl group that consists of three methyl groups, each separately attached to a central (tertiary) carbon. Bioconversion of lignocellulosic biomass to xylitol: An overview cooking, liquid–solid extraction, and filtration in a single step.
The most commonly observed phenolic derivatives in the lignocellulosic acid hydrolysate include 4-hydroxy benzoic acid, ferulic acid, and guaiacol (Mussatto and Roberto, ).